Objective To preliminarily explore the infection status and molecular typing of Borrelia burgdorferi in rodents in parts of Fujian province. Methods Rodents were captured by the cage method in 5 counties (cities), i.e., Shaowu, Gutian, Shishi, Minhou, and Changle from April 2017 to June 2018. Nucleic acids were then extracted from rodent tissue samples (e.g., liver and kidney). After that, the fragments of 5S-23S rRNA were amplified by nested PCR, and sequencing results were analyzed by MEGA 6.0 software. Results A total of 192 rodents were captured, of which 81 were commensal rodents, accounting for 42.19% (81/192), and 111 wild rodents, accounting for 57.81% (111/192). Among all the rodents, four were detected to be positive for Lyme, accounting for 2.08% (4/192). Of the Lyme-positive rodents, one was commensal rodent, accounting for 1.23% (1/81) of all the commensal ones, and three wild rodents, accounting for 2.70% (3/111). Bioinformatics analysis revealed that SW14 from Shaowu and SS53 from Shishi were both B. valaisiana. The gene sequences of the two strains were identical or highly similar to those of B. valaisiana isolate R48 (EU160458.2) and B. valaisiana isolate 11 (JX888445.1), respectively. The gene sequences of both the SW15 from Shaowu and the GT38 from Gutian were different from those of above-mentioned B. valaisiana. Conclusion Rodents carry B. burgdorferi in the five surveyed counties (cities). The genotype (i.e., B. valaisiana) is consistent with that in the surrounding provinces.

Faunal records of the genus Eulaelaps distributed in mainland China were reviewed, a total of 19 species of the genus have been described including a new record species ( E. oudemansi) recorded here in China. A key to Chinese known species of the genus Eulaelaps is given. This study provides a basis for the faunal study and classification of the genus Eulaelaps in China.

Objective To investigate Leptospira serovars in Fujian province, China, and to provide a scientific basis for the prevention and control of leptospirosis. Methods Five investigation sites were established according to the epidemic situation of this disease. In order to determine the main serovars of Leptospira carried by humans and host animals, Leptospira was cultured with blood, kidney, or urine collected from rats, pigs, and frogs. The level of anti-Leptospira antibody was determined in human and rat sera. Serovars and infection status of Leptospira were analyzed based on these studies. Results The investigation of hosts showed that the population density of rodents was 5.83% in the open air of the main epidemic area. Main species were Rattus tanezumi, R. losea, and Niviventer fulvescens, which accounted for 29.87%, 28.19%, and 17.11%, respectively. Leptospira was first isolated from N. coxingi in Changtai county. The positive rate of serum antibody in rats was 11.04%. Autumnalis, Australis, and Grippotyphosa were three of the dominant serotypes in rats. The detection rate of serum antibody in humans was 8.25%. Autumnalis, Pyrogenes, Hebdomadis, and Australis were dominant serotypes in humans. Conclusion The serovars of Leptospira carried by rats and humans are complex in Fujian, which means leptospirosis surveillance should be well performed.

Objective To study the taxonomy of the genus Haemolaelaps from China. Methods The study was conducted by the morphological taxonomy and comparative morphology of the organism. Results Twenty-three species of the genus Haemolaelaps from China were recorded, and a new key to the species of the genus Haemolaelaps was established. Conclusion This study provides the baseline data for the distribution and classification of the genus Haemolaelaps from China.

【Abstract】 Objective To understand the pattern of epidemic areas of hemorrhagic fever with renal syndrome (HFRS) in Fujian and to provide scientific basis for its prevention and control.  Methods Rats were captured in springs and autumns by live snap method, and its density and specie composition were investigated. The virus antigen from rodent lungs was isolated and identified. Results The dominant species in residential areas was Rattus norvegicus in Rattus type areas and the mixed?type area, which density were 4.91% and 5.73% at home respectively. While in the field, it was Apodemus agrarius with the average density of 12.95%, which was obviously higher in autumn and winter than that in spring. R.norvegicus mainly carried with Type Ⅱ virus, and Ap.agrarius with Type Ⅰvirus. A44 and R50 virus strain were isolated from them, respectively. Conclusion R.norvegicus was still the main host of HFRS in Fujian. Ap.agrarius was the main host in the field in the diversity epidemic area. It should strengthen the surveillance and the control of HFRS in main epidemic areas.

Objective To investigate the distribution of gamasid mites and chigger mites on rodents in Sanduao island of Fujian.Methods Rodents were captured alive with cages,and the mites parasitized on the rodent were collected and identified.Results 6769 gamasid mites collected belonged to 4 family,8 genus and 13 species,and 31 580 chigger mites collected belonged to 1 family,2 genus and 6 species.The density of gamasid mites parasitized on Niniventer fulvescens was the highest,and the dominant mite species were Laelaps turkestanicus and L.fukienensis.The infested rate and the infested index of chigger mites to Rattus rattoides and N.fulvescens was all higher,and the dominant species parasitized on R.rattoides was Leptotrombidium scutellare and that on N.fulvescens was L.pallidum.The infested rate of gamadis mites to R.rattoides in bushes and mountainous regions was 86.67% and the infested index was 9.99,which were higher compared to the infested rate of 68.57% and the infested index of 6.86 in paddy fields.The infested rate of chigger mite to R.rattoides in bushes and mountainous was 93.14% and the infested index was 128.30,which were higher compared to the infested rate of 36.90% and the infested index of 29.03 in paddy fields. L.echidninus and L.nuttalli appeared in the whole year without obvious seasonal changes. L.fukienensis, L.turkestanicus, L.scutellare, L.pallidum,Doloisia guangdongensis and L.allosetnm were all active in winter and spring while Haemolaelaps chinensis only appeared in winter,and L.deliense appeared in summer and autumn when the temperature was comparatively higher.Conclusion 13 species of gamasid mites and 6 species of chigger mites were found on the rodents in Sanduao island,and the changes of species composition and population density was affected by host,habitats and seasons.